What is Semaglutide?
Semaglutide is a GLP-1 receptor agonist originally developed for type 2 diabetes management. Known commercially as Ozempic (diabetes) and Wegovy (weight management), it mimics the incretin hormone GLP-1, regulating appetite, insulin secretion, and gastric emptying. In clinical trials, participants lost an average of 15–17% of body weight over 68 weeks at the 2.4 mg maintenance dose.
How to Reconstitute Semaglutide
Lyophilized semaglutide requires reconstitution with bacteriostatic water. Inject the BAC water slowly against the glass wall of the vial — never directly onto the powder. Swirl gently until fully dissolved (typically 2–3 minutes). The solution should be clear and colorless; discard if cloudy or contains particles.
Recommended BAC Water by Vial Size
- 2 mg vial → 1 mL BAC water (2 mg/mL)
- 3 mg vial → 1 mL BAC water (3 mg/mL)
- 5 mg vial → 2 mL BAC water (2.5 mg/mL)
- 10 mg vial → 3 mL BAC water (3.33 mg/mL)
Titration Schedule
Semaglutide requires slow dose escalation over 16–20 weeks to minimize gastrointestinal side effects such as nausea, vomiting, and diarrhea. Rushing this schedule is one of the most common mistakes and significantly increases side effect severity.
- Weeks 1–4: 0.25 mg weekly (starting dose)
- Weeks 5–8: 0.5 mg weekly
- Weeks 9–12: 1.0 mg weekly
- Weeks 13–16: 1.7 mg weekly
- Weeks 17+: 2.4 mg weekly (maintenance)
Injection Technique
Semaglutide is administered subcutaneously, typically in the abdomen, thigh, or upper arm. Rotate injection sites to prevent lipodystrophy. Inject at the same time each week for consistent blood levels. Allow the alcohol swab to dry completely before injecting — wet alcohol stings.
Storage Guidelines
Unreconstituted powder: freezer at −20°C for long-term, or fridge at 2–8°C for shorter periods. Once reconstituted: refrigerate at 2–8°C and use within 28 days. Never freeze reconstituted solution — freeze-thaw cycles break peptide bonds and reduce potency. Protect from light.
Common Side Effects
The most frequently reported side effects include nausea (especially during dose escalation), constipation, diarrhea, and injection site reactions. These typically diminish as the body adjusts. Serious but rare side effects include pancreatitis and gallbladder problems — seek medical attention for severe abdominal pain.
This information is for educational and research purposes only. Semaglutide is a prescription medication. Always consult a licensed healthcare provider before beginning any protocol.